Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . Specialized filters allow for IR fluorescence. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. Discover our high performance reader. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. This protocol gives the cells viability and confluence to be monitored. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate cells Stimulate cells 3. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. $352. FOR HTRF CERTIFIED READER. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Make sure the setup for Tb Cryptate is used. The sample is then added and incubated 1. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. CLARIOstar is equipped with a specific optical device which enables the measurement of. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. Make sure the setup for Eu3+ Cryptate is used. It also integrates with the. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. FOR HTRF CERTIFIED READER. Even if not listed, some. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For more information. and Canaa -8-963-4567. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. 5 μL of Binding buffer 2X. Rating: 5. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). After defining the objectives and their locations in Gen5: 1. For instance, when assay volumes are. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. To perform the assay, use white plate only. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. EX: HTRF Emission range. Typical applications The HTRF cartridge provides optimal performance for key applications. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Alpha Technology: The VICTOR Nivo system. Manufacturer. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. The donor and acceptor can be used to label a variety of biomolecules, for. FOR HTRF CERTIFIED READER. VICTOR® Nivo™ multimode plate reader 2 | For research use only. Assay setup for a 384-well low-volume plate. FLUOstar Omega Filter-based multi-mode microplate reader . For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. Read & analyse. In this case the Z´(pronounced Z prime) value comes to mind. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. For more information about HTRF. Microplate readers EnVision ® Multimode Plate Reader VICTOR® Nivo™ EnSight® Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ® The EnVision ® multimode plate reader provides exceptional speed,. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. It guarantees high level of cAMP accumulation in the cells. Make sure the setup for Tb Cryptate is used. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Endpoint mode experiments measure each well of the microplate only once. FOR HTRF CERTIFIED READER . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about HTRF. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. 6% for 1,408 wells and a Z’ value of 0. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. Plate 25 μL of cells in. HTRF is compatible with a large range of available microplate readers. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. 5% and an increased Z’ value of 0. FlexStation® 3 Multi-Mode Microplate Reader. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. If not, our scientific team will help you choose the instrument that best suits your needs and budget. For more. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. Plate 25 μL of cells in. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. For more information about HTRF. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. Plate 25 μL of cells in. Quantify up to 10 analytes per well. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. But sometimes these parameters are not sufficient enough. 0. Configure your microplate reader. use of a wide variety of plates or sample matrix formats. 10 µL Standard or Sample. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. HTRF is compatible with a large range of available microplate readers. . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Incubate 4h at RT. 5 hours before a further 3 washing steps. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more information about HTRF. For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). HTRF plate reader. CLARIOstar is equipped with a specific optical device which enables the measurement of. Fluorescence intensity – Fluorescence intensity is one of the most. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Dual-Luciferase® (DLR™) gene assays. At that stage, the analyte is sandwiched between the 2 antibodies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Seal the plate and incubate 3 hours at RT . 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Incubate 2 h at RT. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. When MikroWin opens, if you already have a pre-existing template for. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. • HTRF®-Certified Reader. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Each well requires 10 μL of 4X lysis buffer /well. The assay was prepared in both white and black plates and incubated >18 hours. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. • HTRF®-Certified Reader **. Plate 25 μL of cells in. Plate 25 μL of cells in. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. 10 μL of GLP1 receptor cells were dispensed into wells of. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 68. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Results for HTRF Reader Control Kit with black and white microplates. This protocol gives the cells viability and confluence to be monitored. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. At Cisbio we provide microplates that help you discover better. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. 5 EXAMPLE OF MAP PLATE . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. At Cisbio we provide microplates that help you discover better. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. Partnerships for delivering turn-key solutions. The kit includes everything you need: reagents, buffers and a microplate. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. 5 µL Prostaglandin E2 . The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. NC1286225. For more information. MATERIALS PROVIDED: KIT . microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. For more information about HTRF. VICTOR Nivo Multimode Plate Reader. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It continues to deliver optimal results. Introduction. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. The laser beam passes through the sample well. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF certified . Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Read & analyse. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. These are the different kinetic modes on BMG LABTECH microplate readers. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. 1) . Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Highly compatible plates for HTRF. It guarantees high level of cAMP accumulation in the cells. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The plate was covered and incubated for two hours at room temperature. FOR HTRF CERTIFIED READER. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. The CLARIOstar® Plus is our most flexible multi-mode plate reader. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. It is typically used to determine the concentration of nucleic acids and proteins. Plate 25 μL of cells in. • HTRF®-Certified Reader. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Read & analyse. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. Incubate overnight at RT. Table 1. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Plate 25 μL of cells in. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. Dedicated UV-laser excitation. It enables you to gain insights you couldn’t achieve with detection measurements alone. Push the screw down and turn it clockwise to lock the plate. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Incubate at room temperature. For more information about HTRF. Table 1. HTRF-compatible SpectraMax Multi-Mode Readers. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Incubate overnight at RT. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. For more information about HTRF. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. Add. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Catalog No. Phospho-Rb (Ser780) two-plate assay protocol. FOR HTRF CERTIFIED READER. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). HTRF-specific. For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more ® information about HTRF compatible readers and for set-up recommendations. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. Read & analyse. Make sure the setup for Tb3+ Cryptate is used. Eu cryptate emission level). FOR HTRF CERTIFIED READER For more information about HTRF. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. + 4 µL HTRF pre-mixed antibodies . Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. Simply place the module into one of the five module bays and read the assay. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. Incubate overnight at RT. Make sure the setup for Eu3+ Cryptate is used. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. 1. This protocol, HTS designed, allows. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. HTRF certified . Plate 25 μL of cells in 96 half-well plate in your appropriate medium. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate Type and Read Area should be selected based on the microplate used and wells to be read. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. This protocol, HTS designed, allows miniaturization while. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. COMPONENTS ; STORAGE . Revvity. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . Live Viewer for cell-based assays. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . The FLUOstar Omega is the ideal plate reader for life science applications. Use simpler, faster protocols. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. The VICTOR Nivo is a HTRF certified microplate reader. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. For more information about HTRF. Excitation lasers significantly improve performance and lower. Figure 1: Principle of HTRF Cortisol competitive assay. FOR HTRF CERTIFIED READER. Not for use in diagnostic procedures. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF ® compatible readers and for set-up recommendations, please. 1 – 1. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. We would like to show you a description here but the site won’t allow us. HTRF Setup. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol. The optical systems use two scanningHighly compatible plates for HTRF. Smallest and most affordable multimode plate reader in the industry. These plates can be read on all HTRF certified readers, thus making their implementation in. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. Transcreener® ADP2 FP assay. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Microplate readers are only certified if they. 14 seconds for a 384-well plate. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. HTRF Microplate Readers. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. For more information about HTRF. The adapterResults summary for HTRF IP1 standard curve. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. (donor) of compounds are measured on an HTRF-compatible reader. For more information about HTRF. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized.